U.S. Department of Health and Human ServicesHHS National Institutes of HealthNIH National Center for Advancing Translational SciencesNCATS


With support from the NIH Common Fund in 2015 and as part of the NIH Regenerative Medicine Program, NCATS opened the Stem Cell Translation Laboratory (SCTL) in 2017 with innovative laboratory technologies featuring advanced equipment and resources not available to most laboratories. The focus of the SCTL is to bring iPSC-derived cells closer to clinical applications and serve as a reference center for iPSC research and application. The original goals for the SCTL include:

  1. Establish QC standards to define pluripotency and differentiated cell types
  2. Develop methods to assess heterogeneity and characterize iPSC-derived cells using multi-omics technologies
  3. Develop standardized methods to produce mature and functional cells meeting the QC standards above
  4. Discover, validate, and disseminate small molecule reagents to replace expensive recombinant proteins, xenogeneic material, and undefined media components in cell differentiation protocols


Recently acquired equipment includes:

  • CompacT SelecT system for automated cell culture
  • Illumina Next-Seq 500
  • Single-cell analysis: 10X Genomics Chromium, Fluidigm C1, BioRad ddSEQ, Illumina NextGen Seq 500, Fluidigm Helios quantitative single-cell proteomics, HD Biomark qPCR
  • IncuCyte Zoom real-time, quantitative live-cell imaging
  • Fluidigm CyTOF mass cytometry
  • Maxwell Biosystems MaxOne high-density multi-electrode array
  • Seahorse metabolic analyzer