U.S. Department of Health and Human ServicesHHS National Institutes of HealthNIH National Center for Advancing Translational SciencesNCATS
SCTL scientists are working on various projects to address efficiency, reproducibility, scalability, and other roadblocks in the translation of iPSCs into therapies.

The Induced Pluripotent Stem Cell (iPSC) Portal is a scientific repository provided by the Stem Cell Translation Laboratory (SCTL) at NIH’s National Center for Advancing Translational Sciences (NCATS). The iPSC Portal hosts rigorous protocols and searchable datasets generated by SCTL scientists to serve as a reference resource for validated iPSC growth and differentiation protocols, quality control, experimental workflows, data analysis pipelines, and more. Established technologies include large compound libraries, quantitative high-throughput and high-content screening, robotic automation of cell culture workflows, multi-scale assay development, 3-D bioprinting, next-generation sequencing, and integrated platforms to profile gene and protein expression and measure functional endpoints in standard cultures, as well as on the single-cell level.

Staff

Meet the current SCTL members and learn about projects underway in the lab.

Members from left to right: Juliana Ferreira De Sousa, Yogita Jethmalani (now at NIAID), Hannah Baskir, Carlos Tristan, Vukasin Jovanovic, Kathryn Gerrish, Pinar Ormanoglu, and Yeliz Gedik.

 

Publications

Stress-free cell aggregation by using the CEPT cocktail enhances embryoid body and organoid fitness

Seungmi Ryu 1, Claire Weber 1, Pei-Hsuan Chu 1, Ben Ernest 2, Vukasin M Jovanovic 1, Tao Deng 1, Jaroslav Slamecka 1, Hyenjong Hong 1, Yogita Jethmalani 1, Hannah M Baskir 1, Jason Inman 1, John Braisted 1, Marissa B Hirst 2, Anton Simeonov 1, Ty C Voss 1, Carlos A Tristan 1, Ilyas Singeç 1 1National Center for Advancing Translational…


A defined roadmap of radial glia and astrocyte differentiation from human pluripotent stem cells

Vukasin M. Jovanovic,1,∗ Claire Weber,1 Jaroslav Slamecka,1 Seungmi Ryu,1 Pei-Hsuan Chu,1 Chaitali Sen,1 Jason Inman,1 Juliana Ferreira De Sousa,1 Elena Barnaeva,1 Marissa Hirst,2 David Galbraith,2 Pinar Ormanoglu,1 Yogita Jethmalani,1 Jennifer Colon Mercado,3 Sam Michael,1 Michael E. Ward,3 Anton Simeonov,1 Ty C. Voss,1 Carlos A. Tristan,1 and Ilyas Singeç1,∗∗…


News

Stress-free cell aggregation by using the CEPT cocktail enhances embryoid body and organoid fitness
Embryoid bodies (EBs) and self-organizing organoids derived from human pluripotent stem cells (hPSCs) recapitulate tissue development in a…
A defined roadmap of radial glia and astrocyte differentiation from human pluripotent stem cells
Human gliogenesis remains poorly understood, and derivation of astrocytes from human pluripotent stem cells (hPSCs) is inefficient and…
Nociceptor manuscript featured as the cover of Stem Cell Reports
Cover In this issue, Deng et al. describe an efficient strategy that differentiates human pluripotent stem cells into neural crest…
Cytoprotective and stress-free single cell cloning of human iPSCs
We have a new blog post written by our postdoc Yogita that is featured on Nature Protocol’s Behind…