U.S. Department of Health and Human ServicesHHS National Institutes of HealthNIH National Center for Advancing Translational SciencesNCATS

Single-cell RNA analysis of iPSCs and differentiated progeny

Posted on July 19th, 2018 by claire.malley@nih.gov
Three-dimensional neuronal culture derived from pluripotent stem cells. Green: tyrosine hydroxylase, a marker for dopamine neurons. Red: MAP2 (microtubule associated protein 2), a marker for mature neurons. Blue: nuclear marker 4',6-diamidino-2-phenylindole (DAPI).

Three single-cell systems- 10X Genomics Chromium, BioRad ddSeq, and Fluidigm C1 – are being used to characterize different iPSC lines and differentiated cell types for subsequent Illumina NextSeq RNA sequencing. These projects define gene expression signatures for specific cell types and investigate new genes and pathways that control cell type identities and function. The CompacT SelecT robotic cell culture system provides robust standardization for cell growth and differentiation of large numbers of cell lines across different developmental stages.

Image:┬áThree-dimensional neuronal culture derived from pluripotent stem cells. Green: tyrosine hydroxylase, a marker for dopamine neurons. Red: MAP2 (microtubule associated protein 2), a marker for mature neurons. Blue: nuclear marker 4′,6-diamidino-2-phenylindole (DAPI).

To learn more about ongoing SCTL projects, visit our Projects page.