U.S. Department of Health and Human ServicesHHS National Institutes of HealthNIH National Center for Advancing Translational SciencesNCATS
SCTL scientists are working on various projects to address efficiency, reproducibility, scalability, and other roadblocks in the translation of iPSCs into therapies.

The Induced Pluripotent Stem Cell (iPSC) Portal is a scientific repository provided by the Stem Cell Translation Laboratory (SCTL) at NIH’s National Center for Advancing Translational Sciences (NCATS). The iPSC Portal hosts rigorous protocols and searchable datasets generated by SCTL scientists to serve as a reference resource for validated iPSC growth and differentiation protocols, quality control, experimental workflows, data analysis pipelines, and more. Established technologies include large compound libraries, quantitative high-throughput and high-content screening, robotic automation of cell culture workflows, multi-scale assay development, 3-D bioprinting, next-generation sequencing, and integrated platforms to profile gene and protein expression and measure functional endpoints in standard cultures, as well as on the single-cell level.

Staff

Meet the current members of the SCTL and learn about projects underway in the lab.

Members from left to right, back row: Charles “Pepper” Bonney, Pinar Ormanoglu, Claire Malley, Ilyas Singec, Jaroslav Slamecka, Vukasin Jovanovic, Tao Deng.

Left to right, front row: Robin Yu Chen, Yeliz Gedik, Pei-Hsuan Chu, Seungmi Ryu, Carlos Tristan, Michael Iannotti,  Elena Barnaeva.

Photo: Daniel Soñé Photography, LLC

Publications

Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail

Carlos A Tristan, Hyenjong Hong, Yogita Jethmalani, Yu Chen, Claire Weber, Pei-Hsuan Chu, Seungmi Ryu, Vukasin M Jovanovic, Inae Hur, Ty C Voss, Anton Simeonov, Ilyas Singeç National…

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Single-cell RNA-Seq of hPSC-derived Neural Cell Types Reveals Early Glia-specific Differentiation Trajectories

Poster presented at ISSCR 2022 by Vukasin Jovanovic. The poster is entitled: Single-cell RNA-Seq of hPSC-derived Neural Cell Types Reveals Early Glia-specific Differentiation Trajectories. Authors (underlined, presenting): Vukasin…

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News

Cytoprotective and stress-free single cell cloning of human iPSCs
We have a new blog post written by our postdoc Yogita that is featured on Nature Protocol’s Behind…
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SCTL Scientists identify small-molecule cocktail to improve stem cell use in research and disease treatments
In a series of experiments, SCTL scientists used high-throughput screening to systematically test thousands of compounds and drugs…
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See our new publication on bioRxiv
A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells Yu Chen, Carlos A.…